reference cb190 wild type atcc 29213 parent strain  (ATCC)

Journal:

Article Title: In Vitro Evaluation of CBR-2092, a Novel Rifamycin-Quinolone Hybrid Antibiotic: Studies of the Mode of Action in Staphylococcus aureus ▿

doi: 10.1128/AAC.01649-07

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Table shows the relevant details for a series of derivatives of S. aureus ATCC 29213 (CB190) and RN4220 (CB1244) that exhibit stable resistance to agents of the rifamycin and/or quinolone classes that were isolated and characterized as described below. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Relevant genotype Relevant characteristics Source or reference CB190 Wild type ATCC 29213 parent strain for isogenic strain panel ATCC CB370 rpoB H481Y Spontaneous rifampin-resistant variant of CB190 This work CB808 parC S80F Spontaneous ciprofloxacin-resistant variant of CB190 This work CB809 rpoB H481Y parC S80F Spontaneous ciprofloxacin-resistant variant of CB370 This work CB811 gyrA S84L Spontaneous nadifloxacin-resistant variant of CB190 This work CB812 rpoB H481Y gyrA S84L Spontaneous nadifloxacin-resistant variant of CB370 This work CB814 parC S80F gyrA S84L Spontaneous higher-level ciprofloxacin-resistant variant of CB808 This work CB815 rpoB H481Y parC S80F gyrA S84L Spontaneous higher-level ciprofloxacin-resistant variant of CB809 This work CB1244 Wild type RN4220 parent strain: rsbU agr , restriction negative, methylation plus laboratory strain I. Chopra CB1522 rpoB H481Y Spontaneous rifampin-resistant variant of CB1244 This work Open in a separate window Bacterial strains used in this study Isolation and characterization of resistant mutants.

Techniques: Variant Assay, Methylation

Journal:

Article Title: In Vitro Evaluation of CBR-2092, a Novel Rifamycin-Quinolone Hybrid Antibiotic: Studies of the Mode of Action in Staphylococcus aureus ▿

doi: 10.1128/AAC.01649-07

Figure Lengend Snippet: Characterization of mutants from step selections undertaken with wild-type strains

Article Snippet: Table shows the relevant details for a series of derivatives of S. aureus ATCC 29213 (CB190) and RN4220 (CB1244) that exhibit stable resistance to agents of the rifamycin and/or quinolone classes that were isolated and characterized as described below. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Relevant genotype Relevant characteristics Source or reference CB190 Wild type ATCC 29213 parent strain for isogenic strain panel ATCC CB370 rpoB H481Y Spontaneous rifampin-resistant variant of CB190 This work CB808 parC S80F Spontaneous ciprofloxacin-resistant variant of CB190 This work CB809 rpoB H481Y parC S80F Spontaneous ciprofloxacin-resistant variant of CB370 This work CB811 gyrA S84L Spontaneous nadifloxacin-resistant variant of CB190 This work CB812 rpoB H481Y gyrA S84L Spontaneous nadifloxacin-resistant variant of CB370 This work CB814 parC S80F gyrA S84L Spontaneous higher-level ciprofloxacin-resistant variant of CB808 This work CB815 rpoB H481Y parC S80F gyrA S84L Spontaneous higher-level ciprofloxacin-resistant variant of CB809 This work CB1244 Wild type RN4220 parent strain: rsbU agr , restriction negative, methylation plus laboratory strain I. Chopra CB1522 rpoB H481Y Spontaneous rifampin-resistant variant of CB1244 This work Open in a separate window Bacterial strains used in this study Isolation and characterization of resistant mutants.

Techniques: Selection, Mutagenesis

Journal:

Article Title: In Vitro Evaluation of CBR-2092, a Novel Rifamycin-Quinolone Hybrid Antibiotic: Studies of the Mode of Action in Staphylococcus aureus ▿

doi: 10.1128/AAC.01649-07

Figure Lengend Snippet: Antibacterial activities of CBR-2092 against isogenic Staphylococcus aureus strains bearing combinations of rifamycin or quinolone resistance mutations

Article Snippet: Table shows the relevant details for a series of derivatives of S. aureus ATCC 29213 (CB190) and RN4220 (CB1244) that exhibit stable resistance to agents of the rifamycin and/or quinolone classes that were isolated and characterized as described below. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Relevant genotype Relevant characteristics Source or reference CB190 Wild type ATCC 29213 parent strain for isogenic strain panel ATCC CB370 rpoB H481Y Spontaneous rifampin-resistant variant of CB190 This work CB808 parC S80F Spontaneous ciprofloxacin-resistant variant of CB190 This work CB809 rpoB H481Y parC S80F Spontaneous ciprofloxacin-resistant variant of CB370 This work CB811 gyrA S84L Spontaneous nadifloxacin-resistant variant of CB190 This work CB812 rpoB H481Y gyrA S84L Spontaneous nadifloxacin-resistant variant of CB370 This work CB814 parC S80F gyrA S84L Spontaneous higher-level ciprofloxacin-resistant variant of CB808 This work CB815 rpoB H481Y parC S80F gyrA S84L Spontaneous higher-level ciprofloxacin-resistant variant of CB809 This work CB1244 Wild type RN4220 parent strain: rsbU agr , restriction negative, methylation plus laboratory strain I. Chopra CB1522 rpoB H481Y Spontaneous rifampin-resistant variant of CB1244 This work Open in a separate window Bacterial strains used in this study Isolation and characterization of resistant mutants.

Techniques:

Journal:

Article Title: In Vitro Evaluation of CBR-2092, a Novel Rifamycin-Quinolone Hybrid Antibiotic: Studies of the Mode of Action in Staphylococcus aureus ▿

doi: 10.1128/AAC.01649-07

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Interestingly, the doubling time of the terminal day 26 isolate (CB1884), which bears five mutations ( rpoB R484H , gyrA Δ L520 , gyrA S84L , parC R236 duplication, and parC H103Y ), is 65 min and is thus significantly longer than that of parent strain CB190 (ATCC 29213) (38 min).

Techniques: Variant Assay, Methylation

Journal:

Article Title: In Vitro Evaluation of CBR-2092, a Novel Rifamycin-Quinolone Hybrid Antibiotic: Studies of the Mode of Action in Staphylococcus aureus ▿

doi: 10.1128/AAC.01649-07

Figure Lengend Snippet: Characterization of mutants from step selections undertaken with wild-type strains

Article Snippet: Interestingly, the doubling time of the terminal day 26 isolate (CB1884), which bears five mutations ( rpoB R484H , gyrA Δ L520 , gyrA S84L , parC R236 duplication, and parC H103Y ), is 65 min and is thus significantly longer than that of parent strain CB190 (ATCC 29213) (38 min).

Techniques: Selection, Mutagenesis

Journal:

Article Title: In Vitro Evaluation of CBR-2092, a Novel Rifamycin-Quinolone Hybrid Antibiotic: Studies of the Mode of Action in Staphylococcus aureus ▿

doi: 10.1128/AAC.01649-07

Figure Lengend Snippet: Antibacterial activities of CBR-2092 against isogenic Staphylococcus aureus strains bearing combinations of rifamycin or quinolone resistance mutations

Article Snippet: Interestingly, the doubling time of the terminal day 26 isolate (CB1884), which bears five mutations ( rpoB R484H , gyrA Δ L520 , gyrA S84L , parC R236 duplication, and parC H103Y ), is 65 min and is thus significantly longer than that of parent strain CB190 (ATCC 29213) (38 min).

Techniques: